Journal: eLife

Article Title: Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration

doi: 10.7554/eLife.45853

Figure Lengend Snippet: ( A ) Angular histograms showing the distribution of polarization angles from siVinculin transfected cells expressing Vinculin-Full-Length-GFP (n = 4 images, two independent experiments) or Vinculin-T12-GFP (n = 4 images, two independent experiments). ( B ) Polarity index box plots of siVinculin transfected cells non-infected (n = 4 images, two independent experiments) or infected with Vinculin-Full-Length-GFP (n = 4 images, two independent experiments) or Vinculin-T12-GFP (n = 4 images, two independent experiments). p-values from unpaired t-test.

Article Snippet: Recombinant DNA reagent , Vinculin-T12 mutant-GFP , This paper , Addgene plasmid #46266 pEGFPC1/GgVcl 1–1066 T12 mutant; RRID: Addgene_46266 , Lentiviral vector expressing vinculin T12 mutant tagged with GFP.

Techniques: Transfection, Expressing, Infection

Journal: eLife

Article Title: Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration

doi: 10.7554/eLife.45853

Figure Lengend Snippet: ( A ) Angular histograms showing the distribution of polarization angles from siControl and siWNT5a transfected cells either non-infected (n = 21–23 images, from six independent experiments) or expressing Vinculin-Full-Length-GFP (n = 9–11 images, from six independent experiments) or Vinculin-T12-GFP (n = 9–11 images, from six independent experiments). ( B ) Polarity index box plots of siControl and siWNT5a transfected cells either non-infected (n = 21–23 images, from six independent experiments) or Vinculin-Full-Length-GFP (n = 9–11 images, from six independent experiments) or Vinculin-T12-GFP (n = 9–11 images, from six independent experiments). p-values from unpaired t-test. ( C ) siControl and siWNT5a transfected HUVECs expressing Vinculin-Full-Length-GFP and Vinculin-T12-GFP. Nucleus labeled with Dapi, Golgi apparatus with GM130 and adherens junctions with VE-Cadherin. Scale bar, 20 µm. ( D ) Quantification of cell perimeter (%) composed of linear (blue), serrated (red) and reticular (green) in siControl and siWNT5a transfected cells expressing either Vinculin-Full-Length-GFP (n = 16 and 10 cells, respectively, from three independent experiments) or Vinculin-T12-GFP (n = 20 and 21 cells, respectively, from three independent experiments). Data are mean ± SD and p-values from unpaired t-test.

Article Snippet: Recombinant DNA reagent , Vinculin-T12 mutant-GFP , This paper , Addgene plasmid #46266 pEGFPC1/GgVcl 1–1066 T12 mutant; RRID: Addgene_46266 , Lentiviral vector expressing vinculin T12 mutant tagged with GFP.

Techniques: Transfection, Infection, Expressing, Labeling

Journal: eLife

Article Title: Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration

doi: 10.7554/eLife.45853

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Vinculin-T12 mutant-GFP , This paper , Addgene plasmid #46266 pEGFPC1/GgVcl 1–1066 T12 mutant; RRID: Addgene_46266 , Lentiviral vector expressing vinculin T12 mutant tagged with GFP.

Techniques: Generated, Recombinant, Plasmid Preparation, Expressing, Mutagenesis, Clone Assay, Binding Assay, Sequencing, Bicinchoninic Acid Protein Assay, Activation Assay, In Situ, Western Blot, Purification, Molecular Weight, Marker, SYBR Green Assay, Software, Chemotaxis Assay, Migration, Immunostaining, Modification, Staining